Typically a mixture of numerous proteins, for example cell lysate, is used as a sample with ion exchangers. Different types of proteins will bind to the coated magnetic particles with affinities that depend on both the conditions used and the types and number of individual charged groups. The QuickPick™ CM and DEAE kits are based on magnetic cellulose particles coated with carboxymethyl (CM) groups or diethylaminoethyl (DEAE) groups. weak anion exchange kit for proteins, 48 preps.weak anion exchange kit for proteins, 8 preps. weak cation exchange kit for proteins, 48 preps.weak cation exchange kit for proteins, 8 preps.The principle of the mechanism is based on the interaction between a metal ion coordinated to a covalently bound chelating ligand and a His-tag protein. Immobilized metal ion affinity chromatography (IMAC) was originally introduced as a method for group separation, but today it represents one of the most important tools for the single-step purification of proteins based on polyhistidine tags. The QuickPick IMAC kits provide a fast and simple means for recombinant histidine-tagged (His-tag) protein purification based on metal affinity using Ni2+. metal affinity kit for proteins, 48 preps. metal affinity kit for proteins, 8 preps.The number of preparations for a kit is calculated for a "standard" sample size and may this vary depending on the actual sample sizes used. Most of the QuickPick™ kits are scalable ("SML" = Small-Medium-Large) meaning that the amounts of reagents used are directly proportional to the sample size.
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